Biotechnology and Genetics

Stock Identification

Over successive generations, distinct fish populations have adapted to fit and prosper in particular niches in their ecosystems. These specialized characteristics are frequently expressed as unique patterns in their genetic code. The Molecular Genetics Lab at the Pacific Biological Station utilizes microsatellite DNA and major histocompatibility complex (MHC) genetic variation to examine differences in fish populations for ecological and conservation reasons as well as to assist in fisheries management.

To date, hundreds of distinct fish stocks (primarily Pacific salmon) have been examined resulting in the most comprehensive set of microsatellite DNA baseline data for fisheries in the world. Current and pending applications include the management of Chinook salmon in the lower Fraser River and the Skeena River, Sockeye salmon in Barkley Sound and the Fraser, Stikine and Nass rivers, Coho salmon in the Fraser River and lower Strait of Georgia, Fraser River steelhead, and Atlantic salmon in Newfoundland. The results from our study of MHC genetic variation are very promising and this technology is currently being applied to salmonid species as well as other marine species such as Pacific Ocean perch, Pacific herring, and Atlantic cod.

In addition to our team of highly trained professionals, the Molecular Biology Lab offers state of the art PCR (polymerase chain reaction) technology as well as Denaturing Gradient Gel Electrophoresis (DGGE) technology (a process developed in the Molecular Biology Lab) to facilitate the processing of DNA samples. In addition to stock identification applications, this technology is relevant to genetic broodstock development and the development of highly sensitive and specific DNA probes.

For further information
Molecular Genetics Laboratory and Data Holdings.

Contacts:
Dr. Terry Beacham
Tel: (250) 756-7149
FAX: (250) 756-7053
E-mail: terry.beacheam@dfo-mpo.gc.ca

Ruth Withler
Tel: (250) 756-7148
FAX: (250) 756-7053
E-mail: ruth.withler@dfo-mpo.gc.ca

John Candy
Tel: (250) 756-7224
FAX: (250) 756-7053
E-mail: john.candy@dfo-mpo.gc.ca